The interaction between clomifene citrate ( CC) and deoxyribonucleic acid ( DNA) was studied by fluorescence spectroscopy using ethidium bromide ( EB) as a fluorescence probe. 以溴化乙锭为荧光探针,利用荧光光谱法研究了枸橼酸氯米芬与脱氧核糖核酸(DNA)之间的相互作用。
Ethidium Bromide Fluorescence Probe on the Interaction between Dinuclear Macrocyclic Polyamine Zn(ⅱ) Complex and DNA 双大环多胺Zn(Ⅱ)配合物及其与DNA作用的溴乙锭荧光探针
Using ethidium bromide ( EB) as a fluorescent probe, the binding of PB with DNA was studied. 以溴化乙锭作为与DNA结合的荧光探针,考察PB对其结合体系的影响。
Application of ethidium bromide in capillary DNA biosensor 溴化乙锭在毛细管DNA传感器中的应用
Effect of ethidium bromide and actinomycin D on high resolution chromosome G-banding 溴化乙锭及放线菌素D对高分辨染色体的作用
Amplified IPCR products were evaluated by1.8% agarose gel electrophoresis and ethidium bromide staining. 扩增产物在1-8%的琼脂糖凝胶上进行电泳分离,溴化乙锭染色。
Methods: The genome DNA of 7 ordinary species Monascus including M. purpureus and a strain of Aspergillus terreus were extracted by CTAB. The content of DNA was assayed doubly by fluorescence intensity of ethidium bromide ( EB) and spectrometer. 方法:以CTAB法提取紫色红曲霉等7种红曲霉和1株土曲霉的基因组DNA;溴化乙啶荧光强度和分光光度双重测定法确定DNA浓度;
A novel fluorimetric method has been developed as a new in vitro method for preliminary screening of metallic complexes as potential antitumour or anticancer agents and for further approach of action mechanism using ethidium bromide as a fluorescent probe. 提出一种抗癌配合物体外初步筛选及作用机制研究的荧光新方法.对本室所合成的十余种平面型新钯配合物作为非顺铂抗癌药物进行了体外筛选及其与DNA结合作用的研究。
OBJECTIVE To set-up safe alternatives to ethidium bromide ( EB). 目的建立比溴化乙锭(EB)更为安全的核酸染色方法。
The results show that the component with ethidium bromide inserting DNA in tumoral chromatin ( TC) increases by 4.86% than that in normal chromatin ( NC). 结果表明,NC中溴化乙锭插入DNA的成分比TC增加了486%;
METHODS By utilizing acridine orange/ ethidium bromide double fluorescent dye staining to detect the apoptosis and by using MTT to detect the growth inhibition; 方法通过吖啶橙/溴乙锭双荧光染色法检测细胞凋亡,并通过MTT法检测细胞生长抑制;
METHODS: The DNA of WKY rats and SHR was amplified with RAPD-PCR using 100 single primers. The PCR products were resolved with electrophoresis on agarose gel and stained with ethidium bromide. 方法:运用100条引物对WKY和SHR的DNA进行RAPD-PCR扩增,琼脂糖凝胶电泳观察。
Mechanism of ethidium bromide fluorescence enhancement in organic solvent and on binding to DNA EB在有机溶剂和结合DNA时荧光增强机理的研究
Methods: DNA damage was measured by the loss of fluorescence after DNA binding to ethidium bromide and by the increased DNA mobility in agarose gel electrophoresis. 方法:通过DNA结合溴乙锭后的荧光损失和DNA琼脂糖凝胶电泳的迁移率来测定DNA的损伤程度。
The amount of transferred RNA was estimated using the ethidium bromide staining method. 样本加样RNA量用溴化乙锭染色法评估。
Agarose gel electrophoresis, ethidium bromide displacement assay, multi-angle laser light scattering, transmission electron microscopy were used to examine self-assembling between polymer and plasmid from various aspects. 为了考查新聚合物和质粒形成的复合物的自聚过程,琼脂糖凝胶电泳,溴乙锭荧光发射,多角度激光散射和透射电镜从不同的方面显示了聚合物和质粒DNA之间的作用和质粒的缩合。
Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe 溴化乙锭探针研究劳氏青莲与脱氧核糖核酸的相互作用
Study on Genie Mutation Induced by Ethidium Bromide in Dark Variant of Luminous Bacteria 溴化乙锭诱导发光细菌暗变株回复突变的研究
Methods Using MTT assay, flow cytometry, Acridine orange and Ethidium bromide staining, and Western blotting analysis, the effects of celecoxib on the proliferation and apoptosis of HT-29 cells as well as its related mechanism were studied. 方法采用噻唑蓝(MTT)比色法,流式细胞仪(FCM)、吖啶橙/溴化乙啶染色结合荧光显微镜、Western印迹法等技术,研究塞来昔布对HT29细胞增殖抑制的影响。
Ethidium bromide ( EB) ( 0. 6 ug/ ml) can strongly induce dark variant ( S1) reversion luminescence, cause reversion mutation and significantly increase reversion rate. 溴化乙锭(EB)(0.6μg/ml)在24h内能强烈地诱导S1回复发光,引起S1的回复发光突变,显著提高了S1的回复发光突变率。
The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide ( EB) as a probe. 以溴化乙锭(EB)为荧光探针,研究了7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用。
The live apoptotic cells were stained with trypan blue, acridine orange/ ethidium bromide ( AO/ EB). Fixed apoptotic cells were stained with May Grunwald Giemsa ( MGG), HE, observed with transmission electron microscope, and detected with TUNEL method. 进行凋亡活细胞的台盼蓝、吖啶橙/溴化乙啶(AO/EB)染色,凋亡细胞固定后的May-Grunwald-Giemsa(MGG)、HE染色和透射电镜观察,并进行TUNEL法凋亡检测。
New Application of Ethidium Bromide: A Novel Fluorescence Probe for Homogeneous Detection of Hydroxy Radical 溴化乙锭的新应用:均相检测羟自由基的新型荧光探针
Using ethidium bromide as a fluorescence probe, the binding mode of the lanthanum(ⅲ) complex with calf-thymus DNA was studied spectroscopically. 用溴化乙锭作为荧光探针,还研究了La(Ⅲ)-L配合物与小牛胸腺DNA的相互作用的过程。
The double fluorescence stain with acridine orange and ethidium bromide was performed to detect the cell vitality. 采用吖啶橙、嗅乙啶双荧光染色观察细胞活率;
The spectroscopy interactions between berberine and DNA, ethidium bromide and DNA are studied by UV-absorption, fluorescence, circular dichroism spectroscopy and agarose gel electrophoresis methods. 利用荧光光谱、紫外-可见吸收光谱、圆二色谱、琼脂糖凝胶电泳方法研究了小檗碱(berberine)和溴化乙锭(EB)与ct-DNA的光谱学作用。
In case of using agarose gel electrophoresis with ethidium bromide ( EB) staining which was traditionally used in RAPD, both methods generally showed simple profiles with less than 10 bands. )为材料,比较了随机扩增多态DNA(RAPD)和DNA扩增指纹(DAF)方法。用RAPD的琼脂糖胶电泳和溴乙锭染色,RAPD和DAF谱一般不足10条带。
Apoptosis rate was observed with ethidium bromide/ acridine orange ( EB/ AO) staining. 溴乙啶/丫啶橙荧光染色观察细胞凋亡率。
Results These mutants were cross resistant towards chemically unrelated compounds such as ethidium bromide and rhodamine. 结果对ANC强抗性突变株DM104对化学特性不相关的化合物如溴化乙锭和诺丹明产生交叉抗性。